MECHANICAL AND STRUCTURAL PROPERTIES OF COLLAGEN NANOFRIBROUS LAYERS UNDER SIMULATED BODY CONDITIONS

Authors

  • Jitka Říhová Czech Technical University in Prague, Faculty of Mechanical Engineering, Department of Mechanics, Biomechanics and Mechatronics, Technická 4, 166 07 Prague 6, Czech Republic
  • Tomáš Suchý Czech Academy of Sciences, Institute of Rock Structure and Mechanics, V Holešovičkách 94/41, 182 09 Prague 8, Czech Republic
  • Lucie Vištejnová Charles University, Medical Faculty in Pilsen, Biomedical Center, alej Svobody 1655/76, 323 00 Pilsen, Czech Republic
  • Lukáš Horný Czech Technical University in Prague, Faculty of Mechanical Engineering, Department of Mechanics, Biomechanics and Mechatronics, Technická 4, 166 07 Prague 6, Czech Republic
  • Monika Šupová Czech Academy of Sciences, Institute of Rock Structure and Mechanics, V Holešovičkách 94/41, 182 09 Prague 8, Czech Republic

DOI:

https://doi.org/10.14311/APP.2019.25.0073

Keywords:

Collagen, fibroblasts, osteoblasts, mechanical properties, structural properties, biodegradation

Abstract

The theme of this paper is the analysis of mechanical and structural properties of nanofibrous COL under simulated body conditions and in the presence of osteoblasts and dermal fibroblasts. COL were prepared by electrostatic spinning of 8wt% collagen type I dispersion with 8wt% (to COL) of PEG in phosphate buffer/ethanol solution (1/1vol). The stability of COL was enhanced by means of cross-linking with EDC and NHS at a molar ratio of 4:1. COL were exposed in culture medium for 21 days and human SAOS-2 human dermal fibroblasts and osteoblasts were cultured therein for 21 days as well. The cell culture on COL was assessed by fluorescence microscopy and metabolic activity. Then the metabolic activity of both cell types grown on COL and PS were measured after 1, 7, 14 and 21 days using the Alamar Blue assay method. Mechanical properties were determined using an tensile test. The influence of the cell activity on secondary structure of COL was verified by IR spectroscopy. Furthermore, the influence of cells on COL was evaluated by SEM.

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Published

2019-12-06

Issue

Vol. 25 (2019): 17th YOUTH SYMPOSIUM ON EXPERIMENTAL SOLID MECHANICS

Section

Articles

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